Effect of febuxostat treatment on ROS production and mitochondrial membrane potential (ΔΨm). a ROS staining was performed using DCFH-DA. Left: representative images of ROS staining; right: ROS fluorescence intensity. b Changes in mitochondrial membrane potential (ΔΨm) are indicated by JC-1 staining; left: Red fluorescence represents the mitochondrial aggregate form of JC-1, indicating intact mitochondrial membrane potential. Green fluorescence represents the monomeric form of JC-1, indicating dissipation of ΔΨm. Right: ratio of red/green fluorescence intensity. Both ROS and ΔΨm staining were observed with a laser confocal microscope (×400) (Bar 50 µm). All values indicate mean ± SD. **P < 0.01 vs. Con, ##
P < 0.01 vs. H/R.