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Table 1 Metabolites that distinguish colon cancer cell lines from ovarian cancer cell lines

From: Metabolic signatures differentiate ovarian from colon cancer cell lines

Metabolic group/metabolites Ovarian-colon Levels in ovary HCT15_HCT116 SKOV3_OVCAR3
p value p value p value
Amino acid metabolism
Citrulline 5.48 × 10−7 Higher 0.0433 0.0003
Cystathionine 5.96 × 10−11 Higher 0.1051 0.0676
Dimethylarginine (SDMA + ADMA) 2.08 × 10−7 Higher 0.2176 0.0545
Histidine 1.61 × 10−7 Higher 0.1230 0.6965
Isoleucine 1.13 × 10−9 Higher 0.2475 0.0003
Methionine 5.96 × 10−11 Higher 0.7959 0.0343
Methionine sulfoxide 1.79 × 10−9 Higher 0.0011 0.0021
N-acetylmethionine 1.79 × 10−9 Higher 0.4813 1.0000
N-acetylputrescine 1.54 × 10−7 Lower 0.2176 0.2133
N6-acetyllysine 4.17 × 10−10 Higher 0.0015 0.0676
Phenylacetylglycine 1.52 × 10−7 Lower 0.1431 0.0043
Phenylalanine 5.96 × 10−11 Higher 0.5288 0.0031
Tyrosine 5.96 × 10−11 Higher 0.2799 0.0434
Valine 5.96 × 10−11 Higher 0.7959 0.0009
Carbohydrate
Isobar: ribulose 5-phosphate, xylulose 5-phosphate 0.000172 Higher 0.0412 0.0003
Mannose-6-phosphate 1.54 × 10−7 Higher 0.6230 0.0005
UDP-glucuronate 5.96 × 10−11 Higher 0.0052 0.3599
Cofactors and vitamins
Thiamin (Vitamin B1) 1.54 × 10−7 Higher 0.0101 0.0044
Energy
Fumarate 2.08 × 10−7 Higher 0.0068 0.0343
Malate 1.63 × 10−5 Higher 0.6305 0.1728
Succinate 5.96 × 10−11 Higher 0.6842 0.0003
Lipids
1-Arachidonoylglycerophosphoethanolamine 1.13 × 10−9 Higher 0.2475 0.1011
1-Docosahexaenoylglycerophosphoethanolamine 3.77 × 10−5 Higher 0.9118 0.0434
1-Palmitoylglycerophosphoethanolamine 0.000128 Higher 0.1230 0.0085
1-Palmitoylglycerophosphoinositol 1.16 × 10−8 Higher 0.7959 0.4598
1-Stearoylglycerophosphoethanolamine 5.49 × 10−6 Higher 0.0892 0.0044
1-Stearoylglycerophosphoinositol 1.24 × 10−5 Higher 0.0756 0.2743
2-Arachidonoylglycerophosphoethanolamine 8.28 × 10−9 Higher 0.9705 0.0085
2-Docosahexaenoylglycerophosphoethanolamine 1.15 × 10−5 Higher 0.0433 0.0031
2-Linoleoylglycerophosphoethanolamine 1.75 × 10−6 Higher 0.5966 0.1728
Butyrylcarnitine 4.53 × 10−6 Lower 0.2176 0.0003
Deoxycarnitine 0.000023 Lower 0.0003 0.5726
Dihomo-linoleate (20:2n6) 5.96 × 10−11 Higher 0.6842 0.2370
Dihomo-linolenate (20:3n3 or n6) 5.96 × 10−11 Higher 0.1431 0.0062
Glycerol 3-phosphate (G3P) 5.96 × 10−11 Higher 0.5787 0.0266
Linoleate (18:2n6) 5.96 × 10−11 Higher 0.6305 0.0831
Mead acid (20:3n9) 5.96 × 10−11 Higher 0.2799 0.0021
Myo-inositol 5.96 × 10−11 Higher 0.6842 0.0062
Scyllo-inositol 5.96 × 10−11 Higher 0.7959 0.6965
Nucleotide
Guanosine 5′- monophosphate (5′-GMP) 6.89 × 10−7 Higher 0.0185 0.6334
Peptide
Alanylleucine 1.79 × 10−9 Higher 0.3527 0.0003
Glycylglycine 5.96 × 10−11 Higher 0.0524 0.2743
Glycylleucine 5.96 × 10−11 Higher 0.0007 0.1220
Leucylaspartate 5.96 × 10−11 Higher 0.7394 0.0545
Leucylglutamate 2.28 × 10−7 Higher 0.2475 0.0005
Leucylglycine 5.78 × 10−9 Higher 0.2176 0.0205
Phenylalanylglycine 5.96 × 10−11 Higher 0.0005 0.0044
Phenylalanylserine 7.22 × 10−8 Higher 0.1051 0.0031
Prolylalanine 2.68 × 10−9 Higher 0.5288 0.6334
Prolylglutamate 5.96 × 10−11 Higher 0.0068 0.0545
Prolylglycine 5.96 × 10−11 Higher 0.2799 0.0676
Serylleucine 3.99 × 10−9 Higher 0.1903 0.0031
Seryltyrosine 1.61 × 10−7 Higher 0.4359 0.9654
Tyrosylglycine 2.38 × 10−10 Higher 0.0029 0.0044
Valylaspartate 9.51 × 10−8 Higher 0.0433 0.0031
Valylglycine 7.15 × 10−10 Higher 0.0355 0.8968
Valylleucine 1.16 × 10−8 Higher 0.3527 0.0014
Xenobiotics
Erythritol 4.07 × 10−8 Higher 0.1230 0.0009
Unknown
X – 11,677 8.28 × 10−9 Higher 0.0021 0.1728
X − 11,687 5.96 × 10−11 Higher 0.0068 0.8968
X − 11,787 2.71 × 10−5 Lower 0.3527 0.0831
X − 14,056 8.28 × 10−9 Higher 0.4813 0.7618
X − 14,603 4.11 × 10−6 Higher 0.0068 0.0067
X − 14,949 7.06 × 10−5 Lower 0.0007 0.0545
X − 15,546 5.96 × 10−11 Higher 0.0089 0.6334
X − 17,115 1.49 × 10−7 Higher 0.0866 0.6965
X − 19,411 1.53 × 10−7 Higher 0.2116 0.2370
  1. Sixty-seven metabolites were identified that significantly differentiated colon cancer cell lines from ovarian cancer cell lines according to following criteria: significant difference between ovarian and colon cell lines (p < 2.22 × 10−4 for comparison of HCT116 + HCT15 vs. OVCAR3 + SCOV3), but no differences between cell lines of the same origin, (p > 2.22 × 10−4 for HCT116 vs. HCT15 and OVCAR3 vs. SCOV3). P values were calculated with metaP server using the Mann–Whitney test.