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Table 1 Metabolites that distinguish colon cancer cell lines from ovarian cancer cell lines

From: Metabolic signatures differentiate ovarian from colon cancer cell lines

Metabolic group/metabolites

Ovarian-colon

Levels in ovary

HCT15_HCT116

SKOV3_OVCAR3

p value

p value

p value

Amino acid metabolism

Citrulline

5.48 × 10−7

Higher

0.0433

0.0003

Cystathionine

5.96 × 10−11

Higher

0.1051

0.0676

Dimethylarginine (SDMA + ADMA)

2.08 × 10−7

Higher

0.2176

0.0545

Histidine

1.61 × 10−7

Higher

0.1230

0.6965

Isoleucine

1.13 × 10−9

Higher

0.2475

0.0003

Methionine

5.96 × 10−11

Higher

0.7959

0.0343

Methionine sulfoxide

1.79 × 10−9

Higher

0.0011

0.0021

N-acetylmethionine

1.79 × 10−9

Higher

0.4813

1.0000

N-acetylputrescine

1.54 × 10−7

Lower

0.2176

0.2133

N6-acetyllysine

4.17 × 10−10

Higher

0.0015

0.0676

Phenylacetylglycine

1.52 × 10−7

Lower

0.1431

0.0043

Phenylalanine

5.96 × 10−11

Higher

0.5288

0.0031

Tyrosine

5.96 × 10−11

Higher

0.2799

0.0434

Valine

5.96 × 10−11

Higher

0.7959

0.0009

Carbohydrate

Isobar: ribulose 5-phosphate, xylulose 5-phosphate

0.000172

Higher

0.0412

0.0003

Mannose-6-phosphate

1.54 × 10−7

Higher

0.6230

0.0005

UDP-glucuronate

5.96 × 10−11

Higher

0.0052

0.3599

Cofactors and vitamins

Thiamin (Vitamin B1)

1.54 × 10−7

Higher

0.0101

0.0044

Energy

Fumarate

2.08 × 10−7

Higher

0.0068

0.0343

Malate

1.63 × 10−5

Higher

0.6305

0.1728

Succinate

5.96 × 10−11

Higher

0.6842

0.0003

Lipids

1-Arachidonoylglycerophosphoethanolamine

1.13 × 10−9

Higher

0.2475

0.1011

1-Docosahexaenoylglycerophosphoethanolamine

3.77 × 10−5

Higher

0.9118

0.0434

1-Palmitoylglycerophosphoethanolamine

0.000128

Higher

0.1230

0.0085

1-Palmitoylglycerophosphoinositol

1.16 × 10−8

Higher

0.7959

0.4598

1-Stearoylglycerophosphoethanolamine

5.49 × 10−6

Higher

0.0892

0.0044

1-Stearoylglycerophosphoinositol

1.24 × 10−5

Higher

0.0756

0.2743

2-Arachidonoylglycerophosphoethanolamine

8.28 × 10−9

Higher

0.9705

0.0085

2-Docosahexaenoylglycerophosphoethanolamine

1.15 × 10−5

Higher

0.0433

0.0031

2-Linoleoylglycerophosphoethanolamine

1.75 × 10−6

Higher

0.5966

0.1728

Butyrylcarnitine

4.53 × 10−6

Lower

0.2176

0.0003

Deoxycarnitine

0.000023

Lower

0.0003

0.5726

Dihomo-linoleate (20:2n6)

5.96 × 10−11

Higher

0.6842

0.2370

Dihomo-linolenate (20:3n3 or n6)

5.96 × 10−11

Higher

0.1431

0.0062

Glycerol 3-phosphate (G3P)

5.96 × 10−11

Higher

0.5787

0.0266

Linoleate (18:2n6)

5.96 × 10−11

Higher

0.6305

0.0831

Mead acid (20:3n9)

5.96 × 10−11

Higher

0.2799

0.0021

Myo-inositol

5.96 × 10−11

Higher

0.6842

0.0062

Scyllo-inositol

5.96 × 10−11

Higher

0.7959

0.6965

Nucleotide

Guanosine 5′- monophosphate (5′-GMP)

6.89 × 10−7

Higher

0.0185

0.6334

Peptide

Alanylleucine

1.79 × 10−9

Higher

0.3527

0.0003

Glycylglycine

5.96 × 10−11

Higher

0.0524

0.2743

Glycylleucine

5.96 × 10−11

Higher

0.0007

0.1220

Leucylaspartate

5.96 × 10−11

Higher

0.7394

0.0545

Leucylglutamate

2.28 × 10−7

Higher

0.2475

0.0005

Leucylglycine

5.78 × 10−9

Higher

0.2176

0.0205

Phenylalanylglycine

5.96 × 10−11

Higher

0.0005

0.0044

Phenylalanylserine

7.22 × 10−8

Higher

0.1051

0.0031

Prolylalanine

2.68 × 10−9

Higher

0.5288

0.6334

Prolylglutamate

5.96 × 10−11

Higher

0.0068

0.0545

Prolylglycine

5.96 × 10−11

Higher

0.2799

0.0676

Serylleucine

3.99 × 10−9

Higher

0.1903

0.0031

Seryltyrosine

1.61 × 10−7

Higher

0.4359

0.9654

Tyrosylglycine

2.38 × 10−10

Higher

0.0029

0.0044

Valylaspartate

9.51 × 10−8

Higher

0.0433

0.0031

Valylglycine

7.15 × 10−10

Higher

0.0355

0.8968

Valylleucine

1.16 × 10−8

Higher

0.3527

0.0014

Xenobiotics

Erythritol

4.07 × 10−8

Higher

0.1230

0.0009

Unknown

X – 11,677

8.28 × 10−9

Higher

0.0021

0.1728

X − 11,687

5.96 × 10−11

Higher

0.0068

0.8968

X − 11,787

2.71 × 10−5

Lower

0.3527

0.0831

X − 14,056

8.28 × 10−9

Higher

0.4813

0.7618

X − 14,603

4.11 × 10−6

Higher

0.0068

0.0067

X − 14,949

7.06 × 10−5

Lower

0.0007

0.0545

X − 15,546

5.96 × 10−11

Higher

0.0089

0.6334

X − 17,115

1.49 × 10−7

Higher

0.0866

0.6965

X − 19,411

1.53 × 10−7

Higher

0.2116

0.2370

  1. Sixty-seven metabolites were identified that significantly differentiated colon cancer cell lines from ovarian cancer cell lines according to following criteria: significant difference between ovarian and colon cell lines (p < 2.22 × 10−4 for comparison of HCT116 + HCT15 vs. OVCAR3 + SCOV3), but no differences between cell lines of the same origin, (p > 2.22 × 10−4 for HCT116 vs. HCT15 and OVCAR3 vs. SCOV3). P values were calculated with metaP server using the Mann–Whitney test.