A heat map of the metabolite profiles. a
Colour coding displays differences between cell lines: green indicates lower levels and red indicates higher levels of metabolite intensity (z-scored data). Samples are indicated by blue color gradation for colon cancer cell lines (HCT15, dark blue; HCT116, light blue), and red and orange color gradation for ovarian cancer cell lines (OVCAR3, red; SKOV3, orange). Metabolites that significantly differentiate colon from ovarian cancer cell lines are framed. The blue frame indicates metabolites detected at higher levels in colon cancer cell lines; the red frames indicate metabolites observed at higher levels in ovarian cancer cell lines. b Exemplified model of patterns investigated across the metabolites for identification of molecules differentiating colon from ovarian carcinomas.