Effect of regional anaesthesia on opioid induced hyperalgesia in diabetic mice. The actions of levobupivacaine sciatic nerve block on perioperative sufentanil induced hyperalgesia were evaluated by three behavioural tests: a von frey; b hot plate; c guarding pain behaviour. Diabetic control group 4 (C): no sufentanil, no levobupivacaine sciatic block, plantar incision; diabetic OIH group 5 (S): sufentanil, no levobupivacaine sciatic block, plantar incision; diabetic OIH + RA group 6 (S + RA): sufentanil, levobupivacaine sciatic block, plantar incision. *P < 0.05 compared group 5 to group 4 (control); +
P<0.05 compared group 6 to group 4. AN after naloxone, BN before naloxone, B basal, C control, D day, H hour, g gram, PI plantar incision, RA regional anaesthesia, S sufentanil, s second. Data presented as mean ± SD.