Effect of regional anaesthesia on opioid induced hyperalgesia in non-diabetic mice. The actions of levobupivacaine sciatic nerve block on perioperative sufentanil induced hyperalgesia were evaluated by three behavioural tests: a time course of mechanical allodynia (withdrawal threshold to von Frey filament, in g), results expressed as mean ± SD; b time course of heat hyperalgesia (withdrawal latency on hot plate, in s), results expressed as mean ± SD; c guarding pain behaviour (0–24 cumulative pain score). Non-diabetic control group 1 (C): no sufentanil, no levobupivacaine sciatic block, plantar incision; non-diabetic OIH group 2 (S): sufentanil, no levobupivacaine sciatic block, plantar incision; non-diabetic OIH + RA group 3 (S + RA): sufentanil, levobupivacaine sciatic block, plantar incision. Naloxone injection was performed for all groups on D7. *P < 0.05 compared group 2 to group 1 (control); +
P<0.05 compared group 3 to group 1. AN after naloxone, BN before naloxone, B basal, C control, D day, H hour, g gram, PI plantar incision, RA regional anaesthesia, S sufentanil, s second.