The effect of prolonged of warm ischaemic injury on renal function in an experimental ex vivo normothermic perfusion system

Table 3 Histological evaluation assessing tubular dilatation (Tubular Dil), tubular debris, vacuolation, interstitial oedema and glomerular shrinkage in wedge biopsies after 3 h of reperfusion

Histology	15 min	60 min	90 min	120 min
Tubular Dil	1.62 ± 0.33	1.80 ± 0.45	1.65 ± 0.43	1.40 ± 0.44
Tubular debris	1.38 ± 0.34	1.37 ± 0.37	1.80 ± 0.53	1.90 ± 0.58
Vacuolation	2.20 ± 0.10	2.45 ± 0.63*	2.37 ± 0.61*	1.20 ± 0.59
Interstitial oedema	1.35 ± 0.25	1.48 ± 0.34	1.38 ± 0.36	1.35 ± 0.78
Glomerular	1.40 ± 0.32**	1.80 ± 0.30	1.95 ± 0.23	1.58 ± 0.25

Sections were stained with H&E and scored over 10 fields, 0 indicating no abnormalities, 1 mild changes, 2 moderate and 3 severe injury.
Vacuolation (* P = 0.033, Tukey’s post test P < 0.05) 60 min WI versus 120 min WI and 90 min WI versus 120 min WI. Glomerular shrinkage (** P = 0.018, Tukey’s post test P < 0.05) 15 min WI versus 90 min WI. Score shown are mean ± SD.

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