Figure 2

Phospho-Thr¹⁷² AMPK, total AMPK, PPARα, PPARγ, phospho-ACC, total ACC, SREBP-1, and intra-renal lipid levels in the renal cortex of db/m and db/db mice with or without anthocyanin treatment. Protein lysates (30 μg) from the renal cortex were separated by SDS-PAGE and analysed by western blotting. a Representative western blots for phospho-Thr¹⁷² AMPK, total AMPK, PPARα, PPARγ, phospho-ACC, total ACC, SREBP-1, and β-actin. b–f Quantitative analyses for phospho-Thr¹⁷² AMPK/total AMPK (b), PPARα/β-actin (c), PPARγ/β-actin (d), phospho-ACC/total ACC (e), and SREBP-1/β-actin (f). *p < 0.05 and **p < 0.01 vs. db/m control, db/m ATC, and db/db ATC mice. g–i Quantitative analyses of intra-renal NEFA (g), triglyceride (h), and total cholesterol (i) concentrations. *p < 0.05 and **p < 0.01 vs. db/m control, db/m ATC, and db/db ATC mice.