Figure 3
From: Novel xeno-free human heart matrix-derived three-dimensional scaffolds
Viability of cardiac cells within scaffolds. Confocal images of top views of a viable (green Calcein AM) and b dead (red propidium iodide) left ventricular primary cells. Cross-sectional views of c live and d dead left ventricular primary cells. Cross-sectional view of live (green) and dead (red) left ventricular primary cells in Matristem® (e). f–h Confocal image views of 30 stacked z-slices of human cardiomyocytes within human ECM scaffolds showing homogeneous distribution. Cardiomyocytes express red fluorescent protein under control of endogenous Myh6 promoter.