Conditioned media of human CRC epithelial cells and CRC myofibroblasts induced anchorage-independent growth. a Percent efficiency of anchorage independent growth in soft agar assays of murine NIH3T3 cells that overexpressed GFP or KRAS12V by lentiviral transduction, and SW48 and SW620 cells. b Increased anchorage independent growth of malignant NIH3T3-KRAS12V cells with addition of conditioned-media from SW620 cells that were cultured under stressed serum-free versus normal serum containing media or control growth media alone. c Serum-free and serum containing conditioned media from SW620 and CRC MF cells maximally induced anchorage independent growth of non-transformed NIH3T3 cells, respectively. d Relative cytokines levels in SW620 and CRC-MF condition media in the presence and absence of serum. e Secreted CXCL1 and IL8 levels in SW620 and CRC-MF conditioned media in the presence and absence of serum as measured by ELISA. Representative experiments were shown from n ≥3 experiments. P values were as indicated: *p < 0.05, **p < 0.01, ***p < 0.001.