HF–HC–HSD leads to an increase in liver to body weight ratio, insulin resistance and steatohepatitis. Male C57Bl/6 mice were fed with either a high fat diet supplemented with 10% cholesterol and sucrose and fructose in the drinking water, or with a control chow diet for 8, 27, or 49 weeks. Body weight over time (a) and liver to body weight ratio (b) is shown at each time point. Blood glucose (c) over time is shown for each time point. Insulin levels are shown in the chow fed and HFD fed mice both before and 30 min post glucose injection (d). Liver tissue was subjected to (e) Oil-Red-O staining. One representative slide from n = 6/group is shown. Liver triglyceride levels (f) and serum alanine aminotransferase (ALT) levels (g) were measured at each time point. *p < 0.01. n = 6–12/group. #p < 0.01—compared to HFD prior to glucose injection, +p < 0.01, ++p = 0.02—compared to mice fed chow diet prior to glucose injection. n = 6–12/group.