Figure 5
CAFs promote cell mobility and invasion. a The effect of CAFs on cell migration was determined by wound-healing assay. During a period of 48 h, the spreading speed of CAL-27 in CAFs CM along the wound edge was faster than that in NFs and its own CM. b Representative images showed that Tca8113 and CAL-27 in CAFs, NFs and its own CM invaded through the matrigel. The number of invaded tumour cells was quantified in the right panel. Columns, mean of triplicate experiments, P < 0.01. c Expressions of epithelial markers α-catenin, β-catenin and E-cadherin and mesenchymal markers α-SMA and vimentin were compared by western blotting analysis between Tca8113 and CAL-27 in CAFs, NFs and its own CM. β-actin was used as a loading control.