Figure 1

Representative double immunohistochemical images of cN0 oral tongue cancer tissue specimens, which indicate high frequency and distribution of CAFs (a) and low frequency and distribution of CAFs (b). Tumor cell nuclei are stained a brown with the Ki-67 antibody, whereas CAFs are stained a fuchsin color in the cytoplasm by the α-SMA antibody . Magnification is ×200 (a, b). The frequency and distribution of CAFs affect overall survival and disease-free survival. Kaplan–Meier curves with univariate analysis (log-rank) for patients with cN0 oral tongue cancer with a high frequency and distribution of CAFs (n = 95) versus a low frequency and distribution of CAFs (n = 83) with respect to overall survival (c) and disease-free survival (d).