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Figure 4 | Journal of Translational Medicine

Figure 4

From: Excessive proliferation and impaired function of primitive hematopoietic cells in bone marrow due to senescence post chemotherapy in a T cell acute lymphoblastic leukemia model

Figure 4

Primitive hematopoietic cells showed a dramatic cell-cycle activation after therapy ahead of leukemic cells. Cell cycle status of primitive hematopoietic cells and leukemic cells in the BM post therapy was analyzed by Ki-67 and Hoechst staining on flow cytometry. CD45.2+LK+S+ cells and leukemic cells post therapy were sorted and the expression patterns of cell-cycle related genes in these cells were examined. CD45.2+LK+S+ cells from normal control and leukemic cells sorted from leukemia-only mice were used as control for either cell type, respectively. a Representative flow cytometry plots of cell-cycle of CD45.2+LK+S cells in the BM of normal control and the 1-day treated leukemic mice on different days as indicated. b Representative flow cytometry plots of cell-cycle of CD45.2+LK+S+ cells in the BM of normal control and the 1-day treated leukemic mice on different days. c Representative flow cytometry plots of cell-cycle of leukemic cells in the BM of the 1-day treated leukemic mice on different days. df Cell-cycle status of CD45.2+LK+S and CD45.2+LK+S+ cells in the BM of normal control and the 1-day treated leukemic mice on different days (n = 3–7). gi Cell-cycle status of leukemic cells in the BM of the 1-day treated leukemic mice on different days (n = 4–8). jk Gene expression patterns of CD45.2+LK+S+ cells in the BM of normal control and the 1-day treated leukemic mice on the 3rd and 5th day post therapy. ln Gene expression patterns of leukemic cells in the BM of the leukemia-only and the 1-day treated leukemic mice on the 1st, 5th, and 12th day post therapy. All data were presented as mean ± SEM. Statistical significance determined as: *p < 0.05; **p < 0.01; ***p < 0.001.

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