Figure 3

Apoptosis has little effect on changes of LK⁺S⁻/LK⁺S⁺ hematopoietic cells since the recovery phase. a Gating strategy for apoptosis using 7-AAD and Annexin-V staining. The cellular uptake of these dyes discriminated cells in Alive (7-AAD^low Annexin-V^low), Necrosis (Annexin-V^low 7-AAD^high) and Apoptosis (Annexin-V^high 7-AAD^low). b–e Percentages of viable CD45.2⁺LK⁺S⁻ cells (b), apoptotic CD45.2⁺LK⁺S⁻ cells (c), viable CD45.2⁺LK⁺S⁺ cells (d), apoptotic CD45.2⁺LK⁺S⁺ cells (e) in the BM of normal control (shown as N) and the 1-day treated leukemic mice on different days (n = 4–5).