Figure 4

Effect of chemical inhibition of PHD or EGLN in mouse on various blood parameters. a Schematic representation of DHB experiment protocol. PHD inhibition was done by giving DHB (ethyl-3, 4, dihydroxybenzoic acid) treatment in mice. DHB was administered from day 1 to 7. On day 8 mice tail bleeding assay was performed. b Decrease in tail bleeding time of mice treated with DHB in a dose dependent manner. c, d PHD inhibition in mice by chemical DHB treatment leads to increase in total platelet count and its distribution width (considered here as active platelet parameter). Both parameters were assessed through automatic hematology analyzer in mouse whole blood (anticoagulated) as described in methods. e, f ELISA for estimation of HIF-1α and VWF antigen levels in mouse plasma after PHD inhibition. ND not detectable. Data shown as mean ± SEM. *p ≤ 0.05, ***p ≤ 0.001 (n = 6 per group).