Humanized mouse and healthy human donor B cell subset analysis. a–c Representative flow cytometric analysis after gating on human CD45+ CD19+ live cells. The Hu-PBL (a) and alpha3(IV)NC1 collagen-immunized Hu-HSC (c) mice are 14 and 162 days post engraftment, respectively. Percentage of gated cells are indicated. d %CD27+ CD38+ expression on intermediate-to-high CD19+ B cells from splenocytes of Hu-PBL mice engrafted with healthy donor PBL, gated as shown in (a).