Figure 1

Human anti-alpha3(IV)NC1 collagen mAb derived from an immunized Hu-HSC mouse. a Human chimerism and lymphocyte subsets from two immunized Hu-HSC mice 23 weeks after engraftment, calculated as described in “Methods”. Shown are mean (±SD). b Representative flow cytometric analysis, with cell gates indicated at the top and percentage of cells indicated within each marker. c Gene segments encoding heavy and light chain variable (V) regions and sequence of the heavy chain CDR3 (delineated according to the IMGT unique numbering for V-DOMAIN [43]), compared to that of the closest corresponding germline V region gene segment alleles, identified in the IMGT/V-QUEST reference database. Dashes represent sequence identity with mAb.