Figure 4

MEK2 peaks correlated with erlotinib sensitivity in NSCLC cells. a NSCLC cells with different sensitivity to erlotinib were profiled for MEK2 activation by charge-CNIA. A higher MEK2 peak with a pI of 5.92 (“R”) is observed in the intrinsically erlotinib resistant H2122 cells, while a higher MEK2 peak with a pI of 5.98 (“S”) is observed in erlotinib sensitive HCC827 cells. The MEK2 pI 5.98 “S” signal was also decreased after the HCC827 cells were cultured in escalating concentrations of erlotinib which led to acquired resistance to the drug. b Quantitation of relative R and S peaks in the three cell lines [48].