Figure 6From: Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cellsAnti-dsDNA antibodies induced NF-κB activation via PERK-eIF2α-ATF4 ER stress pathway. a Depletion of ATF4 by ATF4 siRNA was confirmed by western blot. b HMCs were stimulated with anti-dsDNA antibodies. NF-κB p65 nuclear translocation was measured by western blot analysis of nuclear protein extract. c ATF4 siRNA significantly inhibited NF-κB p65 nuclear translocation induced by anti-dsDNA antibodies. d–f ATF4 siRNA significantly inhibited the expression of IL-1β, MCP-1 and TNF-α mRNA as measured by qPCR. g–i ATF4 siRNA also significantly inhibited the secretion of IL-1β, MCP-1 and TNF-α in supernatants as measured by ELISA. All experiments were repeated for three times. Data were expressed as mean ± SD, *P < 0.01, versus control group, #P < 0.01, versus scramble siRNA group. CTL Control and anti-dsDNA Abs anti-dsDNA antibodies.Back to article page