Figure 3
From: Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells
IRE1α and ATF6 pathways were not activated by anti-dsDNA antibodies in HMCs. HMCs were stimulated with anti-dsDNA antibodies for 24 h. a The expression of p-IRE1α and ATF6 were measured by western blot. b, c Semi-quantitative analysis showed no significant changes in the expression of p-IRE1α and ATF6. d The expression of CHOP was measured by western blot. e TG treatment significantly increased the expression of CHOP but no significant up-regulation of CHOP was found in MHCs stimulated with anti-dsDNA antibodies. All experiments were repeated for three times. Data were expressed as mean ± SD, *P < 0.05, versus control media. CTL Control, anti-dsDNA Abs anti-dsDNA antibodies and TG thapsigargin.