Figure 7

INF-γ expression in the sections of LLABCs, using IHC staining, at ×200 magnification. Briefly, heat-mediated antigen retrieval was performed using citrate buffer, pH 6 (20 min). The sections were then incubated with polyclonal Abs to INF-γ (Abcam, ab9657) at a concentration of 4 µg/ml for 30 min at RT. Polymeric HRP-linker antibody conjugate was used as secondary antibody. DAB chromogen was used to visualize the staining. The sections were counterstained with haematoxylin. a Low level of INF-γ expression, b high level of INF-γ expression. The H score [% of positive cells × intensity of staining (1–3)] was used to assess the level of expression; low was ≤100 and high was >100. Scoring performed on whole tissue section (>10 HPFs). Itu intratumoural, Str peritumoural.