Figure 7

miR-409-3p targets the 3′-UTR of the oncogene NLK. a The putative binding sites of miR-409-3p in NLK 3′-UTR region were predicted. The matched seed sequences were indicated by vertical lines. b The minimum free energy (mfe) required for RNA hybridization was predicted by RNAhybrid software (mfe: −9.3 kcal/mol). c Schematic graph of the putative binding sites of miR-409-3p in the NLK 3′UTR and the mutation in miR-409-3p-binding sites. d miR-409-3p mimics down-regulated luciferase activities controlled by wild-type NLK 3′UTR (*P < 0.01), but did not affect luciferase activity controlled by mutant NLK 3′UTR. The results are means of three independent experiments ± SD.