Figure 7
From: Different maturation cocktails provide dendritic cells with different chemoattractive properties
Expression and functionality of CCR7 by differently matured DC. a Mature DC were evaluated by flow cytometry for the expression of CCR7. The x-fold increase in MFI over immature DC is show as mean ± SE of 13 different donors. b The ability of mature DC to migrate toward the CCR7 ligands CCL21 was evaluated in a trans-well migration assay. After 3 h incubation at 37°C, DC migrated in the lower chamber were counted by flow cytometry. The results of three experiments are represented as mean ± SE upon normalization to the gold standard DC. *p < 0.05 in the Tukey post hoc test.