Evaluation of chemokine production at the protein level in differently matured DC. a “Wash out” supernatants collected 24 h after seeding mature DC in the absence of exogenous stimuli were used to evaluate chemokine secretion by sandwich ELISA or flow cytometry based plexing. The results are represented as the mean ± SE of 4–7 different donors. b The presence of transmembrane (TM)-CXCL16 on mature DC was evaluated by flow cytometry. The results are shown as mean ± SE of the x-fold increase in MFI over immature DC determined from ten different donors. *p < 0.05 in the Tukey post hoc test.