qPCR evaluation of chemokine gene expression in differently matured DC. The expression levels of chemokine genes in immature DC as well as in DC stimulated with gold standard, alt-1, alt-2 and alt-3 maturation cocktails were evaluated by qPCR. Results were first normalised to the mean expression of two housekeeping genes (GAPDH and HPRT1) and then toward the gold standard DC. The results are represented as the mean ± SE of 9–12 different donors. *p < 0.05, ns p > 0.05 in the Tukey post hoc test.