Effects of DC conditioned supernatants on PBMC from cancer patients. Total PBMC from melanoma or RCC patients were added into trans-well insert (3 μm pores) and allowed to migrate toward DC “wash out” supernatants for 5 h. Migrated cells were evaluated by flow cytometry to determine absolute number and immune composition. a Mean ± SE of the x-fold increase in the migration of the different immune populations over gold standard DC-derived supernatants from 4 to 6 different experiments is shown. b x-fold increase in the HLA-DR MFI of migrated CD14+ monocytes versus the original population. c Gating strategy (insert) and percentage of CD25high CD127low Treg of the original PBMC and among migrated cells shown as percentage of CD4+ T cells or of total leukocytes. *p < 0.05 in the Tukey post hoc test.