Fig. 5

T7-OCT4 vaccine induced tumor-specific immune responses. Mice were i.p. immunized with different vaccines on Day 1, 14, 28 and 42. Three days after last immunization, a: Splenocytes were isolated and total splenocytes were re-stimulated with 10 μg/ml OCT4 antigen for 24 h, and the production of IFN-γ in culture supernatants was measured using ELISA. b: Total splenocytes were labelled with PE-anti-mouse CD8 and FITC-anti-mouse CD3, and the percentages of CD8+/CD3+ T cells in total splenocytes were measured using flow cytometry. c: Tumor-specific in vitro CTL response was measured as described in methods. *, P < 0.01 vs group T7; #, P < 0.01 vs group OCT4. Bars shown are mean ± SD (n = 5)