Fig. 2

Real-time PCR validation of mRNA expression in patients with breast cancer. a, c, e The ratios of CXCL13, GABRP and ESR1 mRNA expression in cancer tissues to their corresponding adjacent normal tissues were calculated after real-time PCR detection and normalization to GAPDH expression. X-axis indicates the ratio of mRNA expression in the cancer tissues to their corresponding adjacent normal tissues and Y-axis indicates the number of the specimens. b, d, f Expression levels of CXCL13, GABRP and ESR1 in young women group (n = 130) and older women group (n = 22) were analyzed by real-time PCR analysis and normalized to GAPDH expression. *P < 0.05, **P < 0.01