Table 1 Oligonucleotide sequences of Notch-1 specific shRNAs
Name | Base sequence |
|---|---|
shRNA1-F | 5′-GATCCCTTTGTTTCAGGTTCAGTATTCTTCCTGTCAGAAATACTGAACCTGAAACAAAGTTTTTG-3′ |
shRNA1-R | 5′-AATTCAAAAACTTTGTTTCAGGTTCAGTATTTCTGACAGGAAGAATACTGAACCTGAAACAAAGG-3′ |
shRNA2-F | 5′-GATCCCGCTGCCTGGACAAGATCAATCTTCCTGTCAGAATTGATCTTGTCCAGGCAGCGTTTTTG-3′ |
shRNA2-R | 5′-AATTCAAAAACGCTGCCTGGACAAGATCAATTCTGACAGGAAGATTGATCTTGTCCAGGCAGCGG-3′ |
shRNA3-F | 5′-GATCCCCGGGACATCACGGATCATATCTTCCTGTCAGAATATGATCCGTGATGTCCCGGTTTTTG-3′ |
shRNA3-R | 5′-AATTCAAAAACCGGGACATCACGGATCATATTCTGACAGGAAGATATGATCCGTGATGTCCCGGG-3′ |
NC-F | 5′-GATCCTTCTCCGAACGTGTCACGTCTTCCTGTCAGAACGTGACACGTTCGGAGAATTTTTG -3′ |
NC-R | 5′-AATTCAAAAATTCTCCGAACGTGTCACGTTCTGACAGGAAGCGTGACACGTTCGGAGAAG -3′ |