Fig. 5

Notch-1 downregulation did not impact on the cell cycle distribution of salivary adenoid cystic carcinoma cells in vitro. Cells were treated as in described in Materials and methods. No significant decreases in PrI were found in the pLenR-Notch-1-shRNA1 stably transfected cells compared with negative or positive control cells (panel d, P _A,C > 0.05, P _B,C > 0.05). Statistical analysis was performed with one-way ANOVA. (PrI, proliferation index value) (a) ACC-M (NC); (b) ACC-M Mock (PC); (c) pLenR-Notch-1-shRNA1