Fig. 4

Notch-1 knockdown suppresses clonogenic growth of salivary adenoid cystic carcinoma cells in vitro. Cells were treated as in described in Materials and methods. Cellular proliferation was evaluated using the MTT assays (a) and colony formation was examined by clonogenic assays (b, c). Data shown in (a) and (b) are expressed as mean ± SD of at least three independent experiments in triplicate. *p _{1, 3} < 0.01, p _{2, 3} < 0.01. (1, ACC-M (NC); 2, ACC-M Mock (PC); 3, pLenR-Notch-1-shRNA1)