Fig. 8
PD-1 is upregulated in CARhi cells with intensities dependent upon stimulation conditions. CD8+ CAR-T cells were co-incubated with a various target cell lines or b CD19+ K562 target cells at various E:T ratios. % PD-1+ among CARhi cells was determined by surface antibody staining. Average values of triplicates are shown with error bars indicating ± 1 s.d.