CARhi and CARlo T cells exhibit distinct functional capabilities. a CARhi and CARlo cells were sorted and subsequently cultured without antigen stimulation or exogenous cytokines. Cytokine production was measured 24 h post sorting. b-d Sorted CARhi and CARlo cells were co-incubated with CD19+ K562 targets without exogenous cytokines and monitored for b viable target-cell count, c viable T-cell count, and d cytokine production after 24 h of co-incubation. Sorted CARlo values in b and c are from single samples due to the rarity of viable cells recovered for this population from cell sorting. For all other samples, average values of triplicates are shown with error bars indicating ± 1 s.d.