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Fig. 10 | Journal of Translational Medicine

Fig. 10

From: Identification and selective expansion of functionally superior T cells expressing chimeric antigen receptors

Fig. 10

TCM-derived CAR-T cells produce more CARhi T cells. a Bulk T-cell–derived CD4+ and CD8+ CAR-T cells were co-incubated with CD19+ K562 target cells without exogenous cytokines, and T-cell subtype distribution was quantified after 24 h. TCM: central memory T cells; TEM: effector memory T cells; TEMRA: effector memory-CD45RA+ T cells; TE: effector T cells; TExh: exhausted T cells. b Bulk- and TCM-derived CD8+ CAR-T cells were co-incubated with CD19+ K562 target cells, and the % CARhi and EGFRt and CD25 expression levels among CARhi cells were quantified. c Both the bulk- and TCM-derived populations separated into CARhi/CD25+ and CARlo/CD25, with the TCM-derived sample showing higher CD25 expression overall. In both samples, a subpopulation of contaminating CAR cells was also CD25+, possibly due to paracrine stimulation from CAR+ cells. d Target cell lysis was monitored over 5 days. Average values of triplicates are shown with error bars indicating ± 1 s.d.

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