Fig. 1
A CARhi/CD25+ population emerges upon antigen stimulation of CAR-T cells. a Schematic of the second-generation anti-CD19 CAR with CD28 co-stimulatory domain; cyto: cytoplasmic domain; EGFRt: truncated EGFR peptide; LTR: long terminal repeats; RRE: Rev responsive element; tm: transmembrane domain; scFv: single chain variable fragment; T2A: Thosea asigna virus 2A peptide; WPRE: woodchuck hepatitis virus posttranscriptional response element. b-e CD8+ T cells expressing various CARs separate into CARhi/CD25+ and CARlo/CD25− populations within 24 h of antigen or CD3/CD28 stimulation. T cells expressing anti-CD19 CARs with b CD28 or c 4-1BB co-stimulation domain or d an anti-CD20 CAR with 4-1BB all result in population polarization upon stimulation with K562 target cells expressing cognate antigens. e CAR–T-cell stimulation with magnetic beads coated with anti-CD3 and anti-CD28 antibodies also yields bifurcated populations