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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Preclinical good laboratory practice-compliant safety study to evaluate biodistribution and tumorigenicity of a cartilage advanced therapy medicinal product (ATMP)

Fig. 5

Tumorigenicity study: Comparison of the huChon spheroid group with the reference groups. Changes of mouse body weight over time normalized to the body weight at implantation of the test item (a). Note: ± 2 SD in (k) covers approx. 95 % of all data in the sample. Dis.: Time point of dissection. Percentage of living animals of the three study groups to show the fraction of mice living for a certain amount of time after implantation (b). Animals of the Caco-2 and NHI/3T3 group were sacrificed just before the planned study time according to the defined break-off criteria. Macroscopic view at the prepared implantation site of one representative mouse of each group before (c–e) and after (f–h) dissection. No tumor but the implanted huChon spheroid was located at the implantation site (f, arrow), whereas tumors were observed in the NIH/3T3 (g) and Caco-2 groups (h). Representative anti-HLA-ABC staining of a section through the implantation site of each group (i–k). The huChon spheroids and the tumors derived from human Caco-2 but as expected not from murine NIH/3T3 cells showed a positive HLA-ABC staining. Bars indicate 200 μm

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