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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Preclinical good laboratory practice-compliant safety study to evaluate biodistribution and tumorigenicity of a cartilage advanced therapy medicinal product (ATMP)

Fig. 3

huChon spheroids, their implantation and the isolation of skin areas in the biodistribution study using a three-part punch. HuChon spheroids in a drop of a syringe ready for implantation after GMP manufacturing (a). Subcutaneous implantation of a single huChon spheroid (arrow) by a micro-spatula into the back of the NSG mouse, 1 cm caudally from the occipital pole (b). The spatula had marks every 0.5 cm to ensure correct implantation. The isolated back skin with the two tattoo points from the former wound edges as orientation were spreaded for punching in a Petri dish (c). Three-part punch, developed for this study (d). Closer look with the labeling of the three skin areas, the inner 4-mm disc has been punched out (e). Representative example of such a punched out 4-mm disc with the spheroid (f, arrow). Scale bars: c: 10 mm, e: 4 mm, f: 1 mm

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