Fig. 2

Flow chart of the tumorigenicity study under GLP conditions. The methods and decisions shown in continuous lines and rectangles were performed within the tumorigenicity study presented here. The rectangle in dashed lines refers to a potential outcome of huChon group that did not occur. Methods shown as a rhombus may have two outcomes. The Roman numerals are used in order to match each step in this flow chart with the corresponding explanation in the “Results” section. ^a(within the topmost rectangle) huChon spheroids from five different patients were implanted, eight mice were treated per patient, 5 spheroids per mouse were implanted and thus 40 mice were analyzed. In two mice of the huChon group a benign tumor was detected. However, both tumors were anti-HLA-ABC negative suggesting that the tumors were spontaneous murine and not derived from human cells. Thus, there was no evidence for increased tumorigenicity after implantation of the huChon spheroids