Fig 1

Morphology, phenotype and pluripotency of human EnSCs. a Cultured EnSCs appear to have stromal cell morphology. b GFP-transfected EnSCs. c Normal chromosomes expressed by cells as measured by karyotype analysis at passage 20. d Mesenchymal stem cell marker expression in EnSCs as measured by flow cytometry. e EnSCs can differentiate into adipocytes (oil red), chondroblasts (alcian blue) and osteoblasts (alizarin red) under standard in vitro differentiating conditions (original magnification, 100x). Scale bars = 200 μm (a, b)