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Table 3 Functional activity of CMV-CTL isolated after three week expansion

From: Assessment of the effector function of CMV-specific CTLs isolated using MHC-multimers from granulocyte-colony stimulating factor mobilized peripheral blood

  G-CSF mobilized Non-mobilized
  Activation marker expression
CD137+ 84.50 % ± 0.42 81.20 % ± 3.54
CD69+ 92.00 % ± 9.33 94.10 % ± 1.98
CD137 + CD69+ 83.70 % ± 0.28 80.80 % ± 0.71
  Cytokine production
IFN-γ 89.93 % ± 4.65 76.50 % ± 14.64
IL-2 0.61 % ± 0.56 1.91 % ± 1.30
IL-10 0.71 % ± 0.84 0.27 % ± 0.41
TNF-α 3.43 % ± 3.69 18.42 % ± 23.85
Granzyme B 55.83 % ± 28.36 92.07 % ± 2.28
  1. Activation marker re-expression and cytokine production in response to antigenic re-challenge was assessed in CMV-CTLs obtained from G-CSF mobilized (n = 3) and non-mobilized (n = 3) PBMCs after 21 day expansion in the presence of the peptide. Percentages were obtained from the CD3+ cell gate