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Table 1 Differentiation status of directly selected CMV-CTL

From: Assessment of the effector function of CMV-specific CTLs isolated using MHC-multimers from granulocyte-colony stimulating factor mobilized peripheral blood

  G-CSF mobilized Non-mobilized
  TN TCM TEM TEMRA TN TCM TEM TEMRA
Before isolation 0.35 % ± 0.07 0.05 % ± 0.07 75.10 % ± 9.90 24.50 % ± 9.76 0.50 % ± 0.87 0.13 % ± 0.23 67.33 % ± 32.66 32.07 % ± 32.98
Positive Fraction 0.50 % ± 0.40 0.93 % ± 1.45 76.77 % ± 16.26 21.87 % ± 17.56 0.00 % ± 0.00 0.03 % ± 0.06 70.27 % ± 32.51 29.73 % ± 32.51
Expanded CMV-CTL 0.30 % ± 0.30 0.00 % ± 0.00 83.8 % ± 13.07 15.90 % ± 12.86 0.13 % ± 0.12 0.07 % ± 0.12 69.77 % ± 20.40 30.03 % ± 20.39
  1. TN, TCM, TEM and TEMRA subpopulations were determined for G-CSF mobilized and non-mobilized samples in PBMCs before CMV-CTL isolation (n = 3), positive fraction of ST+ CMV-CTL isolation (n = 3), and CMV-CTL after 21 day in vitro expansion (n = 3). Percentages were obtained from the CD3 + CD8 + ST+ cell subpopulation