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Table 1 Differentiation status of directly selected CMV-CTL

From: Assessment of the effector function of CMV-specific CTLs isolated using MHC-multimers from granulocyte-colony stimulating factor mobilized peripheral blood

 

G-CSF mobilized

Non-mobilized

 

TN

TCM

TEM

TEMRA

TN

TCM

TEM

TEMRA

Before isolation

0.35 % ± 0.07

0.05 % ± 0.07

75.10 % ± 9.90

24.50 % ± 9.76

0.50 % ± 0.87

0.13 % ± 0.23

67.33 % ± 32.66

32.07 % ± 32.98

Positive Fraction

0.50 % ± 0.40

0.93 % ± 1.45

76.77 % ± 16.26

21.87 % ± 17.56

0.00 % ± 0.00

0.03 % ± 0.06

70.27 % ± 32.51

29.73 % ± 32.51

Expanded CMV-CTL

0.30 % ± 0.30

0.00 % ± 0.00

83.8 % ± 13.07

15.90 % ± 12.86

0.13 % ± 0.12

0.07 % ± 0.12

69.77 % ± 20.40

30.03 % ± 20.39

  1. TN, TCM, TEM and TEMRA subpopulations were determined for G-CSF mobilized and non-mobilized samples in PBMCs before CMV-CTL isolation (n = 3), positive fraction of ST+ CMV-CTL isolation (n = 3), and CMV-CTL after 21 day in vitro expansion (n = 3). Percentages were obtained from the CD3 + CD8 + ST+ cell subpopulation