Fig. 5

CMV-CTL production by prior stimulation, expansion, and subsequent isolation. CMV-CTL percentage in original PBMCs was increased by peptide stimulation and cells were expanded during 21 days in the presence of IL-15 and IL-7. Afterwards, CMV-CTL were isolated using ST. The process was carried out with non-mobilized (n = 3) and G-CSF mobilized (n = 3) PBMCs. (a) Representative histograms of a CMV-CTL expansion and subsequent isolation. (b) Mean yield and purity of ST+ cell selection process for G-CSF mobilized and non-mobilized expanded PBMCs. Memory phenotype of CMV-CTL obtained after isolation, from G-CSF mobilized (c) and non-mobilized (d) samples. Anti-viral function of isolated cells assessed by activation marker expression (e) and cytokine production (f) upon peptide re-challenge