Fig. 3
Differentiation status of CMV-CTL in the original sample, after isolation and after expansion. (a) Mean CD57 expression of the CD3+ cells was analyzed during in vitro culture in G-CSF mobilized (n = 3) and non-mobilized (n = 3) donor samples. Four subpopulations were distinguished based on their CCR7 and CD45RA memory marker expression: TN, TCM, TEM and TEMRA. Percentage of the four differentiation stages was determined for G-CSF mobilized (b) and non-mobilized (c) donor samples. They were analyzed in PBMCs before CMV-CTL isolation (n = 3), positive fraction of ST+ CMV-CTL isolation (n = 3) and CMV-CTL after 21 day expansion (n = 3)