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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Delayed administration of allogeneic cardiac stem cell therapy for acute myocardial infarction could ameliorate adverse remodeling: experimental study in swine

Fig. 6

Y chromosome detection of intracoronary delivered pCSCs. a In order to determine the sensitivity of PCR amplification, pCSCs from a male donor were mixed with 106 pCSCs from a female donor at the indicated ratios. Genomic DNA was extracted and subjected to PCR amplification using Y chromosome specific primers. PCR allowed the detection of male cells with a sensitivity of 100-1000 cells per 106 female cells. b Female pigs were intracoronary injected with male-derived pCSCs. After euthanasia, heart tissue samples were collected for PCR analysis. A representative PCR for each group is shown. As negative and positive controls, the genomic DNA from female and male pCSCs cells were amplified

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