Figure 5
Effect of the FASN inhibitor cerulenin on apoptosis. Effect of cerulenin on apoptosis in ovarian cancer cell lines and ex vivo tumor material. CDDE was used to analyze programmed cell death in the four selected ovarian cancer cell lines, Hey, Heycis, Igrov-1, and Skov-3, and two primary ovarian cancer cell cultures G2 and G3 #1, after administration of 25 μmol/L cerulenin. Caspase activation was increased from 1.5- to 3.3-fold in the ovarian cancer cell lines and from 1.3- to 1.7-fold in the ex vivo cancer cells relative to that (caspase activation induced by cerulenin to 12x100) in healthy tube tissue cells (controls; Ø). Optical density (OD) = 405 nm (490-nm reference wavelength).