Figure 3
Effect of the FASN inhibitors cerulenin and C75 on FASN expression. Effect of the FASN inhibitors cerulenin and C75 on FASN expression of ovarian cancer cell lines and ex vivo tumor material. (a) Immunoblot and quantitative analyses of relative (rel) FASN protein expression of the four selected ovarian cancer cell lines Hey, Heycis, Igrov-1, and Skov-3 when untreated (Ø) or after treatment with cerulenin (cer) or C75. Treatment with 35 μmol/L cerulenin typically markedly reduced FASN protein expression, whereas treatment with 35 μmol/L C75 caused just a slight diminution or no diminution of such expression in the investigated cell lines. (b) Immunoblot and quantitative analyses of relative (rel) FASN protein expression of primary ovarian cancer cell cultures G2, G3 #1, and G3 #2 when untreated (Ø) or after treatment with cerulenin (cer) or C75. As in the cell lines, in two of three cases the inhibitory effect of cerulenin exceeded that of C75.