Effects of LMW-FGF-2 on myofibroblast activation. (A) Representative confocal microscopic images of cardiac myofibroblasts embedded in a collagen gel, following treatments (control, TGFβ1 and LMW-FGF-2 + TGFβ1). Cells were stained for α-SMA (green) and nuclei (DAPI; blue). (B) TGFβ1 increased α-SMA expression while LMW-FGF-2 diminished α-SMA expression. Bars represent mean ± SD; *p < 0.01. (C) α-SMA was labeled with FITC-conjugated antibody and the intensity of a-SMA expression was measured using flow cytometry. TGFβ1 increased the cellular expression of α-SMA and this effect was abolished by LMW-FGF-2.