Figure 1

Comprehensive differential DNA methylation analysis in prakriti. (A) Venn diagram for the identification of prakriti specific methylated probes. The numbers indicated in the black boxes represent the identified 501 mPSRs in which 23, 303 and 175 probes were found to be more methylated in Kapha, Pitta and Vata respectively. (B) Distribution of uniquely significant methylated probes with respect to CpG islands and CpG shores. The probes are classified on the basis of its position from TSS, as upstream promoter (−10Kb to -1Kb), promoter (−1Kb to transcription start site), 5′UTR, gene body (within the genic region), 3′UTR, Downstream (Up to 10Kb from the end of gene coordinates) and Intergenic. (C) Genome wide distribution of 501 mPSRs. Bar height indicate the degree to which each chromosome contains an unexpectedly high number of differentially methylated probes. Bar height is the –log10 values of fisher exact p-value derived from no FDR correction. The height of bars higher than black dashed line was considered to be significant (p ≤ 0.05). (D) Hierarchical cluster analysis of 501mPSR. M-values scale represents the relative methylation. Column represents the samples and rows represented by 501mPSRs. The arrays and probes belonging to Kapha, Pitta and Vata are shown in red, green and blue respectively.