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Table 3 Selected references reporting roles of miRNAs with a high relative recovery during cerebral microdialysis

From: Detection and quantification of microRNA in cerebral microdialysate

MiRNA Author, year Design/model Tissue/material Relation of miRNA to acute cerebral injury
miR-29b Shi et al, 2011 [7] A: 90 min of MCA occlusion in rats (ischemia) A: Brain tissue 24 hours after occlusion Increased miR expression (A+B), repression of the anti-apoptotic protein Bcl2L2, and thereby neuronal cell death (B).
B: Oxygen-glucose deprivation in cortical neuronal cell cultures from rats. B: Cell suspension Pre-treating neuronal cultures with miR-29b inhibitor decreased neuronal cell death (B)
miR-34a Su et al, 2014 [16] A: 15 min of MCA occlusion in mice (preconditioning) A: Microglia extracted ex vivo after 3 days MiR promotes CNS inflammation in microglia by suppressing transcription of the twist2 gene and thereby the anti-inflammatory gene cMaf.
B: Microglia cell culture from mice B: Cell suspension Induced by expression of p53.
Truettner et al, 2013 [15] Stretch injury, cortical neuron cultures from rats Cell suspension MiR promotes apoptosis by inhibiting translation of the anti-apoptotic proteins Bcl2 and XIAP and increasing expression of the apoptotic cytokine Caspase 11.
miR-155 Su et al, 2014 [16] A: 15 min of MCA occlusion in mice. (preconditioning) A: Microglia extracted ex vivo after 3 days Increased miR expression (A) MiR promotes CNS inflammation in microglia by suppressing the anti-inflammatory gene cMaf in microglia (A+B)
B: Microglial cell culture from mice B: Cell suspension Normal cytokine induced expression of miR-155 is suppressed in p53-deficient microglia (B).
Following treatment with INF-γ, the normal expression of IL-1α and IL-1β are suppressed in miR-155 knock out microglia cell cultures (B).
Freilich et al, 2013 [19] LPS stimulation, microglial cell cultures from mice pups Cell suspension Increased miR expression after LPS and thereby activation of several pro-inflammatory pathways.
  1. MCA = middle cerebral artery, CNS = central nervous system, LPS = lipopolysaccharide.