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Table 1 Schematic overview of pre-analytical and analytical setup

From: Detection and quantification of microRNA in cerebral microdialysate

Sample number Sample name Source Micro-dialysis Catheter pore size Perfusion fluid for microdialysis qPCR – number of miRNA
1 RNA MD20 Human brain, total RNA In vitro 20 kDa CNS 377
2 RNA MD100 Human brain, total RNA In vitro 100 kDa HA 3.5% 377
3 RNA REF Human brain, total RNA None NA NA 377
4 H MD100 Healthy human, LP-CSF In vitro 100 kDa HA 3.5% 377
5 H REF Healthy human, LP-CSF None NA NA 377
6 SAH1 MD20A SAH patient 1, EVD-CSF In vitro 20 kDa CNS 754
7 SAH1 MD20B SAH patient 1, EVD-CSF In vitro 20 kDa CNS 754
8 SAH1 MD20C SAH patient 1, EVD-CSF In vitro 20 kDa CNS 754
9 SAH1 MD20D SAH patient 1, EVD-CSF In vitro 20 kDa CNS 754
10 SAH1 REFA SAH patient 1, EVD-CSF None NA NA 754
11 SAH1 REFB SAH patient 1, EVD-CSF None NA NA 754
12 SAH1 REFC SAH patient 1, EVD-CSF None NA NA 754
13 SAH1 REFD SAH patient 1, EVD-CSF None NA NA 754
14 SAH2D3 MD20 SAH patient 2, in vivo MD, day 3 In vivo 20 kDa CNS 377
15 SAH3D3 MD20 SAH patient 3, in vivo MD, day 3 In vivo 20 kDa CNS 377
16 SAH2D9 MD20 SAH patient 2, in vivo MD, day 9 In vivo 20 kDa CNS 377
17 SAH3D9 MD20 SAH patient 3, in vivo MD, day 9 In vivo 20 kDa CNS 377
18 SAH2D3 REF SAH patient 2, EVD-CSF, day 3 None NA NA 377
19 SAH3D3 REF SAH patient 3, EVD-CSF, day 3 None NA NA 377
20 SAH2D9 REF SAH patient 2, EVD-CSF, day 9 None NA NA 377
21 SAH3D9 REF SAH patient 3, EVD-CSF, day 9 None NA NA 377
  1. qPCR, real-time polymerase chain reaction; miRNA, microRNA; MD, microdialysis; CNS, “Central nervous system perfusion fluid” (Mdialysis); HA, human albumin (CLS Behring); ref, reference; NA, not applied; LP-CSF, cerebrospinal fluid obtained by lumbar puncture; SAH, spontaneous aneurysmal subarachnoid hemorrhage; EVD-CSF = cerebrospinal fluid obtained from external ventricular drain.